Molecular biology and biochemistry graduate student Jie Zhai (left) explains a scene for videographer Kai-Jie Wang Dec. 15 inside the Hingorani Laboratory. Wang works for the Journal of Visualized Experiments (JoVE), a peer reviewed, indexed journal devoted to the publication of biological research in a video format. He is filming a project at Wesleyan titled "Application of Stopped-flow Kinetics Methods to Investigate the Mechanism of Action of a DNA Repair Protein."
Zhai and her peers use a KinTek-brand stopped-flow kinetics instrument to monitor the activities of DNA repair proteins in real-time. The JoVE video will explain how the lab uses the instrument so others can develop their own experiments on their own system. The equipment and film were supported by a National Science Foundation (NSF) grant awarded to Manju Hingorani, associate professor of molecular biology and biochemistry.
Molecular biology and biochemistry major Christopher Doucette '11 and BA/MA student F. Noah Biro also are featured in the JoVE video. The filmed experiments explain how they're able to monitor a protein binding with DNA by using a fluorescent reporter molecule.
Biro explains how a chromatography system works in the lab's 'cold room.' Here, he purifies milligram quantities of a protein from E. coli host cells by ion exchange and chromatography. The JoVE film will be released in early 2010. (Photos by Olivia Bartlett Drake)